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针刺对川芎嗪防治豚鼠庆大霉素中毒性耳聋靶向作用的影响郑华平 王 枫 徐泉珍 徐 飞 张国军 王 鹏 指导:王永华 刘金洪 范炳华


  文章编号:0255-2930(2008)07-0515-04

  中图分类号:r 246.81 文献标志码:a 机制探讨

  [摘 要] 目的:探讨治疗庆大霉素中毒性耳聋的有效方法及作用机制。方法:将豚鼠随机分为正常对照组(a组)、模型组(b组)、川芎嗪组(c组)、针刺组(d组)和针刺加川芎嗪组(e组)。c、d、e组分别给予单纯川芎嗪、单纯针刺(穴取“听宫”“翳风”“外关”)和针刺联合川芎嗪治疗。10 d后检测听性脑干诱发电位(abr)ⅲ波反应阈值、corti器部位细胞凋亡及bax、bcl-2蛋白表达。结果:e组abr阈值低于c组(p<0.05),corti器部位细胞凋亡、bax蛋白表达及bax/bcl-2比值均低于c组和d组,bcl-2蛋白表达有所增加。结论:针刺“听宫”“翳风”及“外关”穴对川芎嗪有一定的靶向协同作用,可提高治疗庆大霉素中毒性耳聋的疗效。其作用机制可能与抑制细胞凋亡、下调bax蛋白表达及上调bcl-2蛋白表达有关。

  [关键词] 聋/针灸疗法;庆大霉素类/中毒;川芎嗪/治疗应用;药物协同作用;豚鼠

  effect of acupuncture on target action of ligustrazine in treatment of toxic deafness induced by gentamicin in guinea pigs

  zheng hua-ping1, wang feng1, xu quan-zhen2, xu fei1, zhang guo-jun1, wang peng2, director

  s: wang yong-hua1, liu jin-hong1, fan bing-hua2 (1.deafness rehabilitation institute, 2.college of acupuncture &massage, zhejiang chinesemedical university, hangzhou 350003, china)

  abstract:objective to explore an effectivemethod for treating gentamicin-induced deafness and themechanism.methods guinea pigs were randomly divided into 5 groups: normal control group (group a),model group(group b), ligustrazine group (group c), acupuncture group (group d) and ligustrazine plus acupuncture group (group e).the group c, d and e were treated respectively by simple ligustrazine, simple acupuncture at "tinggong" (si 19), "yifeng" (te 17), and "waiguan" (te 5), and ligustrazine plus acupuncture.ten days la-ter, the auditory brainstem response (abr) thresholds for the wave ⅲ, apoptosis and expressions of bcl-2 and bax proteins in the organ of corti of the guinea pig were detected.results in the group e, the abr threshold was significantly lower than that in the group c (p<0.05), and apoptotic cells, the expression of bax protein and the ratio of bax/bcl-2 were lower than those in the group c and d in the organ of corti, and bcl-2 protein expression was increased.conclusion acupuncture at "tinggong" (si 19), "yifeng" (te 17), and "waiguan" (te 5) has a certain target-synergistic action on ligustrazine and can increase therapeutic effect of ligustrazine on gentamicin-induced deafness, which are possible related with the inhibition of apoptosis, down-regulation of bax expression and up-regulation of bcl-2 expression.

  key words:deafness/am ther;gentamicins/pois;tetramethylpyrazine/ther use;drug synergism;guinea pigs

  近年来,随着各领域靶向性治疗的研究,耳蜗靶向性研究逐渐兴起,运用传统的中医药治疗手段,

  结合现代靶向治疗方法有望成为中医耳聋研究领域的突破点之一。

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