中图分类号：r 246.81 文献标志码：a 机制探讨
[摘 要] 目的:探讨治疗庆大霉素中毒性耳聋的有效方法及作用机制。方法：将豚鼠随机分为正常对照组(a组)、模型组(b组)、川芎嗪组(c组)、针刺组(d组)和针刺加川芎嗪组(e组)。c、d、e组分别给予单纯川芎嗪、单纯针刺(穴取“听宫”“翳风”“外关”)和针刺联合川芎嗪治疗。10 d后检测听性脑干诱发电位(abr)ⅲ波反应阈值、corti器部位细胞凋亡及bax、bcl-2蛋白表达。结果：e组abr阈值低于c组(p<0.05)，corti器部位细胞凋亡、bax蛋白表达及bax/bcl-2比值均低于c组和d组，bcl-2蛋白表达有所增加。结论：针刺“听宫”“翳风”及“外关”穴对川芎嗪有一定的靶向协同作用，可提高治疗庆大霉素中毒性耳聋的疗效。其作用机制可能与抑制细胞凋亡、下调bax蛋白表达及上调bcl-2蛋白表达有关。
effect of acupuncture on target action of ligustrazine in treatment of toxic deafness induced by gentamicin in guinea pigs
zheng hua-ping1, wang feng1, xu quan-zhen2, xu fei1, zhang guo-jun1, wang peng2, director
s: wang yong-hua1, liu jin-hong1, fan bing-hua2 (1.deafness rehabilitation institute, 2.college of acupuncture &massage, zhejiang chinesemedical university, hangzhou 350003, china)
abstract：objective to explore an effectivemethod for treating gentamicin-induced deafness and themechanism.methods guinea pigs were randomly divided into 5 groups: normal control group (group a),model group(group b), ligustrazine group (group c), acupuncture group (group d) and ligustrazine plus acupuncture group (group e).the group c, d and e were treated respectively by simple ligustrazine, simple acupuncture at "tinggong" (si 19), "yifeng" (te 17), and "waiguan" (te 5), and ligustrazine plus acupuncture.ten days la-ter, the auditory brainstem response (abr) thresholds for the wave ⅲ, apoptosis and expressions of bcl-2 and bax proteins in the organ of corti of the guinea pig were detected.results in the group e, the abr threshold was significantly lower than that in the group c (p<0.05), and apoptotic cells, the expression of bax protein and the ratio of bax/bcl-2 were lower than those in the group c and d in the organ of corti, and bcl-2 protein expression was increased.conclusion acupuncture at "tinggong" (si 19), "yifeng" (te 17), and "waiguan" (te 5) has a certain target-synergistic action on ligustrazine and can increase therapeutic effect of ligustrazine on gentamicin-induced deafness, which are possible related with the inhibition of apoptosis, down-regulation of bax expression and up-regulation of bcl-2 expression.......