互联网 qkzz.net
全刊杂志网:首页 > 女性 > 文章正文
刊社推荐

characterization of cprk1, a crp/fnr-type transcriptional regulator of halorespiration from desulfitobacterium hafniense



【关键词】  halorespiration

    laboratory of microbiology, wageningen university and research centre, hesselink van suchtelenweg 4, 6703 ct wageningen, the netherlands

    abstract

    the recently identified cprk branch of the crp (cyclic amp receptor protein)-fnr (fumarate and nitrate reduction regulator) family of transcriptional regulators includes proteins that activate the transcription of genes encoding proteins involved in reductive dehalogenation of chlorinated aromatic compounds. here we report the characterization of the cprk1 protein from desulfitobacterium hafniense, an anaerobic low-g+c gram-positive bacterium that is capable of reductive dechlorination of 3-chloro-4-hydroxyphenylacetic acid (cl-ohpa). the gene encoding cprk1 was cloned and functionally overexpressed in escherichia coli, and the protein was subsequently purified to homogeneity. to investigate the interaction of cprk1 with three of its predicted binding sequences (dehaloboxes), we performed in vitro dna-binding assays (electrophoretic mobility shift assays) as well as in vivo promoter probe assays. our result......

很抱歉,暂无全文,若需要阅读全文或喜欢本刊物请联系《细菌学杂志》杂志社购买。
欢迎作者提供全文,请点击编辑
分享:
 

了解更多资讯,请关注“木兰百花园”
摘自:细菌学杂志
分享:
 
精彩图文


关键字
支持中国杂志产业发展,请购买、订阅纸质杂志,欢迎杂志社提供过刊、样刊及电子版。
关于我们 | 网站声明 | 刊社管理 | 网站地图 | 联系方式 | 中图分类法 | RSS 2.0订阅 | IP查询
全刊杂志赏析网 2017