[摘 要] 观察电针刺激对心脏手术病人心肌细胞热休克蛋白mrna基因表达的影响。28例asd病人,随机分为针麻组(组ⅰ,n=6),针刺加全麻组(组ⅱ,n=10),和全麻组(组ⅲ,n=9),于转流前10 min,停转流,停转流30 min,停转流1 h测定ckmb。结果:①3组病人停转流和转流后1h ckmb均较转流前明显增加,但组ⅲ的升幅明显高于组ⅰ和组ⅱ;②针刺组hsp70mrna表达高于对照组(p70mrna表达作用。
effects of electroacupuncture on myocardial cellular hsp70mrna gene expression in patients undergoing cardiac surgery
wang xiangrui1,zhang tengfei2,ma shuliang1,et al(1. renji hospital affiliated to shanghai second medical university,shanghai 200127;2. teaching and research section of biochemistry,shanghai second medical university)
[abstract] purpose to observe the effect of electroacupuncture on myocardial cellular hsp70mrna gene expression in patients undergoing cardiac surgery(asd).methods 28 asd patients were randomly divided into 3 groups,i,e,acupuncture anesthesia group(groupⅰ,n=6),acupuncture combined with general anesthesia group(group ⅱ,n=10) and general anesthesia group(groupⅲ,n=9).the sample from left atrium at 10 min before cpb,stopping cpb,30 min and 1 h after stopping cpb.extration of total rna from myocardial tissue using reagent kit.the concentration of mrna was measured through rtpcr and ckmb was detected.results after cpb ckmb in the three groups was significantly higher than the value of 10 min before cpb,and ckmb in the group ⅲ was remarkedly higher than the group ⅰ and ⅱ;hsp70mrna expression was found to be higher in the group ⅰ than that of group ⅲ at 30 min and 1 h after stopping cpb.conclusion electroacupuncture can induce the expression of hsp70mrna in ischemic myocardial tissue of patients undergoing cardiac surgery.
[key words] electroacupuncture;heart surgery;rna,massenger/metab;heatshock proteins/metab;myocardium/metab