［主题词］电针；生长结合蛋白-43*；突触膜糖蛋白/代谢；再灌注损伤/针灸疗法effect of electroacupuncture on gap-43 and synaptophysin expression of ischemic brain in the mcao rat
chen yinghui, huang xianfen (department of neurobiology, shanghai medical university, shanghai 200032, china)
［abstract］purpose to observe the effect of electroacupuncture (ea) on growth-associated protein (gap-43) and synaptophysin expression of ischemic brain in the rat by immunohistochemical method. methods the experiment was divided into three groups: sham operation group, ischemia group and ischemia plus ea group. the middle cerebral artery (mca) was occluded for 90 min to result in the local ischemia. ea was applied for one hour immediately after cerebral ischemia. then ea was applied every day till the rats were decapitated after reperfusion for 1,7 and 14 days, respectively. immunohistochemical method was used. results gap-43 and synaptophysin immunoreactive cells expressed mainly in the surrounding cortex of infarction. in the ea group gap-43 and synaptophysin immunoreactive cells after reperfusion for 7 and 14 days were significantly higher than those in the ischemia group (p＜0.05).conclusion ea may increase expression of gap-43 and synaptophysin in the surrounding cortex of infarction, so ea can promote the neuronal sprouting and synaptogenesis after ischemia, and improve the neuronal plasticity.
［key words］electroacupuncture; growth-associated protein*; synaptophysin/metab; reperfusion injury/acup ther