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针刺“四神聪”对睡眠紊乱模型小鼠生理功能的影响高希言 马巧琳 胡 斌


  文章编号:0255-2930(2007)09-0681-03

  中图分类号:r 246.6文献标识码:a

  [摘 要] 目的:探讨针刺“四神聪”穴对睡眠的影响及作用机制。方法:健康小白鼠48只随机分为四神聪组、足三里组、模型组、正常空白对照组,每组12只,前3组反复定时腹腔注射小剂量咖啡因致小鼠睡眠节律紊乱,前2组分别给予针刺“四神聪”、针刺“足三里”治疗。观察各组生活状态、昼夜活动情况,检测脑组织一氧化氮合酶(nos)活性、一氧化氮(no)含量。结果:针刺“四神聪”和“足三里”均可改善睡眠节律紊乱小鼠的一般生存状态、睡眠节律、睡眠质量,但四神聪组优于足三里组;针刺“四神聪”“足三里”均可显著提高小鼠脑组织nos活性及no含量,但“四神聪”组疗效明显优于“足三里”组(p<0.01)。结论:针刺四神聪治疗失眠、调整睡眠及多种精神神经症状,可能部分是通过增强nos活性、提高no含量,促进了脑组织的正常功能而实现的。

  [主题词] 睡眠障碍/针灸疗法;睡眠/针灸效应;一氧化氮合酶/代谢;穴,四神聪;小鼠

  effects of acupuncture at "sishencong" (ex-hn 1) on physiological functions in the sleep disorder model mousegao xi-yan, ma qiao-lin, hu bin (school of acupuncture & massage, henan college of tcm, zhengzhou 450008, china)abstract objective to probe into the effect of acupuncture at "sishencong" (ex-hn 1) on sleep and the mechanism. methods forty-eight white mice were randomly divided into a sishencong group, a zusanli group, a model group and a blank control group, 12 mice in each group. in the mice of the first 3 groups, sleep rhythm disorders was induced by repeatedly regular intraperitoneal injection of small dose of caffeine, and the mice of the first two groups were treated by acupuncture at "sishencong" (ex-hn 1) and "zusanli" (st 36), respectively. living state and, circadian activities were observed, and nitric oxide synthase (nos) activity and nitric oxide(no) content in the brain were detected. results acupuncture at "sishencong" (ex-hn 1) or "zusanli" (st 36) could significantly increase nos activity and no content in the brain, with acupuncture at "sishencong" (ex-hn 1) being significantly better than acupuncture at "zusanli" (st 36) (p<0.01). conclusion acupuncture at sishencong (ex-hn 1) treats insomnia and regulates sleep and many mental and nervous symptoms, which are carried out partially through increasing nos activity and no content, promoting normal function of brain tissues.

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